Identification of microorganisms in wet coffee fermentation Coffea arabica Var Catimor and Castillo in Jardín, Antioquia-Colombia, using culture-dependent methods / Identificación de microorganismos en fermentación húmeda de café Coffea arabica Var Catimor y Castillo en Jardín, Antioquia-Colombia, usando métodos cultivo-dependientes

Background: Mild Colombian coffees are recognized worldwide for their high-quality coffee cup. However, there have been some failures in post-harvest practices, such as coffee grain fermentation. These failures could occasionally lead to defects and inconsistencies in quality products and economic losses for coffee farmers. In Colombia, one of the fermentation methods most used by coffee growers is wet fermentation, conducted by submerging the de-pulped coffee beans for enough time in water tanks to remove the mucilage. Objectives: We evaluated the effect of the water (g)/de-pulped coffee (g) ratio (I: 0/25, II: 10/25, III: 20/25) and final fermentation time (24, 48, and 72 hours) on the total number of microbial groups. We also identified microorganisms of interest as starter cultures. Methods: We used a completely randomized experimental design with two factors; the effect of the water (g)/de-pulped coffee (g) ratio (I: 0/25, II: 10/25, III: 20/25) and final fermentation time (24, 48, and 72 hours), for 9 treatments with two replicates. During the coffee fermentation (1,950 g), the pH and °Brix were monitored. Total counts of different microbial groups (mesophiles, coliforms, lactic-acid bacteria, acetic-acid bacteria, and yeasts) were performed. Various isolates of microorganisms of interest as starter cultures (lactic-acid bacteria and yeasts) were identified using molecular sequencing techniques. Results: 21 lactic-acid bacteria (LAB) isolates and 22 yeasts were obtained from the different mini-batch fermentation systems. The most abundant lactic-acid bacteria species found were Lactiplantibacillus plantarum (46%) and Levilactobacillus brevis (31%). Pichia kluivery (39%) and Torulaspora delbrueckii (22%) were the most abundant yeast species. Conclusion The studied factors did not have effect over the microorganism's development. The identified bacterial and yeasts species have potential as starter cultures for better-quality coffees and in fermentation-related applications.

Antecedentes: Los cafés suaves lavados colombianos son reconocidos a nivel mundial por su buena puntuación sensorial; sin embargo, se han detectado fallas en las prácticas de postcosecha, como lo es la fermentación de los granos de café. Dichas fallas pueden causar defectos y carecer de consistencia en la calidad del producto, ocasionando pérdidas económicas para los caficultores. En Colombia, uno de los métodos más usados por los caficultores es la fermentación húmeda, la cual consiste en sumergir los granos de café despulpado en tanques con agua por un período de tiempo que permita la remoción del mucílago. Objetivos: La presente investigación evaluó la incidencia que tienen la proporción agua/granos despulpados de café (I: 0/25, II: 10/25, III: 20/25) y el tiempo final de fermentación (24, 48 y 72 horas) en el recuento final de grupos microbianos. Por otra parte, se identificaron taxonómicamente microorganismos de interés para su uso como cultivos iniciadores. Métodos: Mini-lotes consistieron en café despulpado (1950 g) puesto en recipientes de plástico abiertos y sumergidos en agua. Se aplicó un diseño experimental completamente aleatorizado de dos factores (proporción agua/ granos de café despulpado y tiempo) a tres niveles, para un total de nueve tratamientos con dos replicas. Durante las fermentaciones de café (1,950 g), el pH y los grados ºBrix, fueron monitoreados. Se realizaron los recuentos totales de los diferentes grupos microbianos: mesófilos, coliformes, bacterias ácido-lácticas, bacterias ácido-acéticas y levaduras. Se identificaron molecularmente diferentes aislados con potencial para ser usados como cultivos iniciadores (bacterias ácido-lácticas y levaduras). Resultados: Los resultados obtenidos mostraron que no hubo diferencia estádisticamente significativa entre los tratamientos aplicados y el recuento final de microorganismos. Un total de 21 aislados de bacterias ácido-lácticas (BAL) y 22 levaduras lograron obtenerse a partir de los diferentes sistemas de fermentación en mini-lote. Las especies de bacterias ácido-lácticas con mayor porcentaje acorde a su identificación taxonómica, corresponden a Lactiplantibacillus plantarum (46%), Levilactobacillus brevis (31%). Las especies de levaduras con mayor porcentaje acorde a su identificación taxonómica corresponden a Pichia kluivery (39%) y Torulaspora delbrueckii (22%). Conclusión Los factores estudiados no afectaron el crecimiento de ninguno de los grupos microbianos presentes en la fermentacion del café. Las especies de microorganismos identificados tienen potencial para se usados como cultivos starter o en aplicaciones dentro de las ciencias de fermentación.

Green coffee bean extract attenuates gentamicin induced acute nephrotoxicity in rats / El extracto de grano de café verde atenúa la nefrotoxicidad aguda inducida por gentamicina en ratas

Gentamicin induced acute nephrotoxicity (GIAN) is considered as one of the important causes of acute renal failure. In recent years' great effort has been focused on the introduction of herbal medicine as a novel therapeutic agent for prevention of GIAN. Hence, the current study was designed to investigate the effect of green coffee bean extract (GCBE) on GIAN in rats. Results of the present study showed that rat groups that received oral GCBE for 7 days after induction of GIAN(by a daily intraperitoneal injection of gentamicin for 7days), reported a significant improvement in renal functions tests when compared to the GIAN model groups. Moreover, there was significant amelioration in renal oxidative stress markers (renal malondialdehyde, renal superoxide dismutase) and renal histopathological changes in the GCBE-treated groups when compared to GIAN model group. These results indicate that GCBE has a potential role in ameliorating renal damage involved in GIAN.

La nefrotoxicidad aguda inducida por gentamicina (GIAN) se considera una de las causas importantes de insuficiencia renal aguda. En los últimos años, el gran esfuerzo se ha centrado en la introducción de la medicina herbal como un nuevo agente terapéutico para la prevención de GIAN. Por lo tanto, el estudio actual fue diseñado para investigar el efecto del extracto de grano de café verde (GCBE) sobre la GIAN en ratas. Los resultados del presente estudio mostraron que los grupos de ratas que recibieron GCBE oral durante 7 días después de la inducción de GIAN (mediante una inyección intraperitoneal diaria de gentamicina durante 7 días), informaron una mejora significativa en las pruebas de función renal en comparación con los grupos del modelo GIAN. Además, hubo una mejora significativa en los marcadores de estrés oxidativo renal (malondialdehído renal, superóxido dismutasa renal) y cambios histopatológicos renales en los grupos tratados con GCBE en comparación con el grupo del modelo GIAN. Estos resultados indican que GCBE tiene un papel potencial en la mejora del daño renal involucrado en GIAN.

Microclimate and development of Coffea canephora intercropped with Carica papaya: measures to mitigate climate change

Intercropped systems with Conilon coffee might provide a better environment for coffee production. The aim of this study was to assess the microclimate and development of Conilon coffee intercropped with papaya trees. Papaya was planted with spacing of 3.20 x 2.40 m. The coffee trees were planted after eight months, with spacing of 3.20 x 1.60 m, in-between papaya trees (in the same row). The measurements were taken 0, 40 and 80 cm away from the coffee plants, both in the north and south direction. Concomitantly, an adjoining full sunlight coffee system (not intercropped) was also assessed. The measurements included atmospheric parameters (temperature, irradiance, and relative humidity) and vegetative parameters for the coffee plants (leaf area, relative chlorophyll index, length of plagiotropic branches, length of orthotopic branches and number of nodes) in three periods of the year. The intercropped system of Conilon coffee and papaya trees led to a decrease in both irradiance and temperature, and higher means of relative humidity during daytime in all the periods assessed, which contributes to a better environment for coffee cultivation. The shadow provided by papaya trees in the coffee plants contributed to a higher leaf area but did not affect neither the growth of both plagiotropic and orthotopic branches, nor the number of nodes and the etiolation. The intercropped system of Conilon coffee and papaya trees may be potentially used as a farming system to mitigate climate change.

Leaf extract of Coffea arabica L. reduces lipid peroxidation and has anti-platelet effect in a rat dyslipidemia model

Abstract This study aimed to evaluate the antioxidant potential of the Coffea arabica Lineu (L.) leaf extract and its effects on platelet aggregation of dyslipidemic rats. The extract was obtained by the percolation of C. arabica L. leaves in hydroethanolic solution 70% (v/v). The mass spectrometry FIA-ESI-MS² suggested the presence of chlorogenic acid, rutin acid, and quinic acid. The DPPH• radicals scavenging capacity was demonstrated (IC50 = 0.06 mg/mL). The extract was administered to rats by gavage (300 mg/kg/day) for 56 days. Dyslipidemia was induced by administering Triton WR-1339 (300 mg/kg body weight) on the 54th day. On day 56, blood was collected by puncturing the abdominal aorta artery and the aortic artery was removed. Lipid profile, markers of renal and hepatic injury, lipid peroxidation, and platelet aggregation tests were carried out. The ingestion of extract reduced the lipid peroxidation (aorta and plasma) and platelet aggregation in dyslipidemic rats. The extract did not affect markers of renal and hepatic function as analyzed in this study, suggesting neither impaired liver nor kidney function in these animals. Therefore, our results demonstrate that the extract of leaves of C. arabica L. show antioxidant potential in vitro and in vivo as well as anti-platelet aggregation in dyslipidemic animals

Applying different infective structures of Beauveria bassiana to Coffea arabica infested with Hypothenemus hampei (Coleoptera: Curculionidae) under field

Coffee is the most appreciated beverage in worldwide; Brazil is the largest producer and exporter of this commodity. Organochlorine endosulfan was banned from the country in 2013 due to its teratogenic agent-related features. Since then, coffee plantations have experienced increased Hypothenemus hampei infestation rates. The aim of the current study is to assess variations in the rates of Coffea arabica fruits brocaded by H. hampei after the application of entomopathogenic fungal species Beauveria bassiana IBCB66. Experiments were carried out with 'Catuaí' and 'Mundo Novo' cultivars between 2018 and 2020, during the borer transit period. Three experiments were carried out based on the application of the aforementioned fungal species on the investigated coffee plant species, both by spraying and sprinkling, at 30-day intervals; 10 fruits were collected per face of each useful plant in each repetition. The experiment has followed a randomized blocks design with five treatments, including the control, and five repetitions, each. Beauveria bassiana Ecobass (IBCB66) wettable powder spray, at the concentration of 2 × 1013·ha­1, was used in experiments I and II. On the other hand, the mix used in experiment III was prepared with blastospores at concentration of 5 × 1012·ha­1 blastospores + 0.1% Silwet. The sprinkling process in all three experiments has used dry aerial conidia at concentration of 2 × 1013·ha­1. Collected data were subjected to analysis of variance (ANOVA), which was followed by Fisher's test at 5% probability level, in the SISVAR software. More than 35,000 fruits were assessed. In addition to variations between experiments, results have evidenced that the rate of brocaded fruits remained high.

Chemical and sensory characteristics in the selection of bourbon genotypes

The evaluation of coffee quality in Brazil for commercialization is conducted mainly through sensory analysis, also known as the "cup test", in which professional tasters evaluate and score various attributes. The adoption of chemical methods could complement the sensory classification of beverages, if correlations between these chemical and sensory analyses exist, making classification less subjective. This work aimed to identify the relationships between the chemical and sensorial traits of coffee-beverage quality and to evaluate the use of these traits as criteria for the selection of Bourbon cultivars. Twenty coffee genotypes from the first three harvests across five municipalities of the state of Minas Gerais, Brazil were evaluated. The genotypic values, predicted for each genotype, were used to determine the index based on the sum of ranks from Mulamba and Mock. The genetic correlations among the evaluated traits were also estimated. The presented evaluations were not able to efficiently detect genetic and phenotypic relationships between the chemical and sensorial characteristics of drink quality, but as selection criteria for generation advancement in the beverage quality, it is possible to use these characteristics. Bourbon Amarelo LCJ 9-IAC, Bourbon Amarelo-Procafé, Bourbon Amarelo-Boa Vista, Bourbon Vermelho-São João Batista, and Bourbon Amarelo-Samambaia were the genotypes with the most promising cup quality in the studied regions. Through the selection of these five genotypes, the selection gain was 1.65% for sensory score for beverage quality, when the interaction among the studied environments was removed. The heritability was 92% for improving this trait.

Exogenous application of salicylic acid to control coffee rust

The exogenous application of salicylic acid can induce plant resistance against pathogens. However, little is known about the potential uses of this bioregulator for controlling coffee diseases. In this study, we assessed the effect of applying salicylic acid (SA – 150 mg L-1) on the management of coffee rust (Hemileia vastatrix) in a 7-year-old coffee plantation with low crop load (651.6 kg ha-1 in 2017). For comparison, plants were sprayed with protectant fungicide (copper hydroxide – CH) and standard fungicides (SF) used by local farmers (boscalid, pyraclostrobin + epoxiconazole, and copper hydroxide). Non-treated plants were included as a negative control. Five monthly applications were performed from November 2016 to March 2017. Rust incidence and severity, defoliation, and growth of plagiotropic branches were evaluated monthly. The activity of catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and total proteins was assessed one day after the first, third, and fifth product applications. Compared to untreated plants, SA reduced the severity and incidence of rust from 36.3 to 54.7%, while CH and SF reduced disease from 31.8 to 54.6% and from 83.8 to 88%, respectively. SA reduced defoliation by 54.1%. SA increased the concentration of CAT, APX, and SOD after the first application. However, this effect was not observed after subsequent applications. Foliar application of SA reduces the severity and incidence of coffee rust and defoliation in plants with a low crop load.

Coffea arabica L: History, phenology and climatic aptitude of the state of São Paulo, Brazil

The genus Coffea belongs to the Rubiaceae family and includes two species with optimum economic performances, Coffea arabica and Coffea canephora. The state of São Paulo is one of the states that produce the species C. arabica in Brazil. Arabica coffee has been of great importance to São Paulo, providing relevant contributions to the historical, political, architectural, gastronomic, touristic, artistic, agricultural, industrial and social sectors since its introduction into the state in the nineteenth century. The agricultural sector includes crops produced by both small farmers and by highly technological agricultural groups. Coffea arabica plants present six phenological phases, all sensitive to changes in temperature and rainfall. In the reproductive phases, the species requires short days, low temperatures and no rainfall, followed by the rainy season. However, the phenological phases of the coffee plants can be harmed or even inhibited by dry or rainy seasons that are too long or too short. In the state of São Paulo, the municipalities of Garça and Franca show optimal coffee productivity, whereas those of Adamantina and Registro are low, and the agricultural aptitudes of these four municipalities show strong relationships with their geographical distributions in the state. Garça and Franca are situated in areas where the predominant dry and rainy seasons favor the occurrence of the phenological phases of arabica coffee plants, whereas Adamantina and Registro are located in areas with long dry and rainy seasons, respectively, characteristics that harm the development of the reproductive phenological phases of this culture.

Agricultural wastes as alternative substrates in the production of conilon coffee seedlings / Resíduos agrícolas como substratos alternativos na produção de mudas de café conilon

Research related to the use of agricultural residues as alternatives to commercial substrates has become fundamental for reducing the production costs of coffee seedlings. The objective of this study was to evaluate the effect of residues of pepper (Piper nigrum) powder, coffee (Coffea) husk, coconut (Cocos nucifera) fiber and pine (Pinus) bark as alternative substrates in the development of coffee (Coffea canephora) seedlings in tubes. The experiment was carried out at the Demuner Seedling Nursery, in the municipality of São Roque do Canaã, Espírito Santo, Brazil. The experimental design was completely randomized, with six treatments: T0- Bioplant® commercial substrate (control); and from T1 to T5, increasing proportions (%) of pepper powder and decreasing coconut fiber and pine bark (0/20/50, 10/15/45, 20/10/40, 30/5/35 and 40/0/30) and fixed proportions of coffee husk (30%). At 120 days after staking, we evaluated the plant height, number of leaves, stem diameter, shoot dry mass and root dry mass. Any of the substrates containing different proportions of agricultural residues can be used as an alternative to the commercial substrate in the production of Conilon coffee seedlings since most of the growth variables evaluated did not differ from the control. Due to the high availability of pepper powder in the region, we recommended the substrate with 40% pepper powder, 30% coffee husk and 30% pine bark

Pesquisas relacionadas ao uso de resíduos agrícolas como alternativas aos substratos comerciais são fundamental para reduzir os custos de produção das mudas de café. O objetivo do trabalho foi avaliar o efeito dos resíduos pó de pimenta (Piper nigrum), casca de café (Coffea), fibra de coco (Cocos nucifera) e casca de pinus (Pinus) como substratos alternativos no desenvolvimento de mudas de café Conilon (Coffea canephora) em tubetes. O experimento foi realizado no viveiro de produção de mudas Demuner, localizado no município de São Roque do Canaã, ES. Foi adotado o delineamento experimental inteiramente casualizado, com seis tratamentos, sendo, T0: Substrato comercial Bioplant® (testemunha); e de T1 ao T5, proporções crescentes de pó-de-pimenta e decrescentes de fibra de coco e casca de pinus (0/20/50; 10/15/45; 20/20; 20/10/40, 30/5/35 e 40/0/30) e proporções fixas de casca de café (30%). Aos 120 dias após estaqueamento avaliaram-se: altura de planta, número de folhas, diâmetro do coleto, massa de matéria seca da parte aérea e massa de matéria seca radicular. Qualquer um dos substratos contendo diferentes proporções de resíduos agrícolas pode ser uma alternativa ao substrato comercial na produção de mudas de café Conilon, já que a maioria das variáveis de crescimento avaliadas não diferiu da testemunha. Em razão da maior disponibilidade de pó-de-pimenta na região, recomenda-se o substrato contento 40% de pó-de-pimenta, 30% de casca de café e 30% de casca de pinus.

Enzyme Production by Induratia spp. Isolated from Coffee Plants in Brazil

Abstract Endophytic fungi belonging to the genus Muscodor now transferred to Induratia are known producers of bioactive volatile organic compounds (VOCs) with many industrial applications. However, the members of this genus have rarely been reported to produce non-volatile metabolites including enzyme. Enzymes of the endophytes are degraders of the polysaccharides available in the host plants and the knowledge of enzyme production by Induratia spp. may provide insights into their possible biotechnological applications. The aim of this study was to evaluate the activity of amylase, cellulase, lipase, pectinase, phytase, protease, endo β-1,4 glucanase and exo β-1,4 glucanase enzymes produced by fungi of the species Induratia coffeana, Induratia yucatanensis and Induratia sp. isolated from organic coffee plants. All Induratia spp. were able to produce the extracellular enzymes cellulase, pectinase, protease, and phytase. Eight fungi were able to produce lipase and four produced amylase. The specific activity of endo β-1, 4 glucanase and exo β-1,4 glucanase enzymes were detected for 9 and 8 endophytic fungi, respectively. This work demonstrated for the first time, the array of enzymes produced by Induratia spp. isolated from Coffea arabica in organic systems in Brazil.

Influence of Meloidogyne incognita race 1 on the development of clones of Coffea canephora, variety "Jequitibá Incaper 8122" / Influência de Meloidogyne incognita raça 1 no desenvolvimento de clones de Coffea canephora, variedade "Jequitibá Incaper 8122"

Root-knot nematode is one of the most important phytosanitary problems for Conilon coffee, as it reduces productivity and is difficult to handle. We aimed at studying the infectivity and damage caused by M. incognita race 1 in the "Jequitibá Incaper 8122" intermediate maturity coffee variety. The experiment was conducted in a greenhouse, in completely randomized design, with five replicates. The clones composing the variety "Jequitibá Incaper 8122" were inoculated with 2,000 eggs + second-stage juveniles of M. incognita race 1. Uninoculated plants were the control. Evaluations were performed 180 days after inoculation, considering the plant height (H), stem diameter (SD), number of leaves (NOL), leaf area (LA), number of plagiotropic branches (NPB), number of nodes (NN), chlorophyll content (CHLO), shoot dry matter (SDM), root fresh matter (RFM), final population (FNP), and reproduction factor (NRF). The nematode reduced NOL in clones 208 and 209, NRF in clones 201, 203, 207 and 208, NN in clones 203, 207, 208 and 209, CHLO in clones 201, 204, 206, 207 and 209, SDM in clones 201, 203, 204 and 205 and RFM in clones 205 and 207. M. incognita race 1 FNP and NRF were larger in clones 208, 201, 207 and 203. Clone 202 had FNP and NRF equal to zero, being immune to the nematode. Clone 206 presented the lowest NRF value among clones parasitized by M. incognita.(AU)

O nematoide-das-galhas é um dos mais importantes problemas fitossanitários para o cafeeiro conilon, por reduzir a produtividade e ser de difícil manejo. Objetivou-se estudar a infectividade e os danos causados por M. incognita raça 1 na variedade de café conilon de maturação intermediária "Jequitibá Incaper 8122". O experimento foi conduzido em casa de vegetação, em DIC, com cinco repetições. Os clones que compõem a variedade "Jequitibá Incaper 8122" foram inoculados com 2.000 ovos + juvenis de segundo estádio de M. incognita raça 1. Plantas não inoculadas constituíram a testemunha. As avaliações foram realizadas 180 dias após a inoculação, sendo avaliados: altura da planta (ALT), diâmetro do caule (DCA), número de folhas (NFO), área foliar (AFO), número de ramos plagiotrópicos (NRP), número de nós (NN), teor de clorofila (CLO), massa seca da parte aérea (MSA), matéria fresca da raiz (MFR), população final (PFN) e fator de reprodução (FRE). O nematoide reduziu o NFO nos clones 208 e 209, NRP nos clones 201, 203, 207 e 208, NN nos clones 203, 207, 208 e 209, CLO nos clones 201, 204, 206, 207 e 209, MSA nos clones 201, 203, 204 e 205 e MFR nos clones 205 e 207. PFN e FRE de M. incognita raça 1 foram maiores nos clones 208, 201, 207 e 203; o clone 202 teve a PFN e a FRE igual a zero, apresentando-se imune ao nematoide. O clone 206 apresentou o menor valor de FRE entre os clones parasitados por M. incognita.(AU)

Vegetative growth and yield of robusta coffee genotypes cultivated under different shading levels / Crescimento vegetativo e produção de genótipos de café robusta cultivados sob diferentes níveis de sombra

The growing in shaded systems is one of the strategies adopted to mitigate the impacts of climate change on coffee trees. However, there are few studies about the effect of shading on the growth and production of clones of robusta coffee (Coffea canephora). Besides that, exist a paradigm in Brazil of that the shading in coffee robusta is synonymous of less yield. Thus, the aim of this work was to evaluate whether C. canephora genotypes subjected to shading increase their yield and growth vegetative under field conditions. The study was performed between September 2013 and July 2017 (four harvests) in Alegre, Espírito Santostate, Brazil. Three genotypes of cultivar Conilon Vitória - Incaper 8142 were evaluated (03V, 06V, and 12V) and four levels of shade: full sunlight (witness) and, low, moderate and, high shade, with 38, 48 and, 68% of real capacity of shade, respectively. The shade was performed using black polyolefin screens; plants growing in full sunlight were only fenced. The tested shade levels affected the growth rate and yield of Robusta coffee plants. The shading caused a greater leaf expansion as compared to the full sunlight, as well as greater etiolation of the plagiotropic and orthotropic branches. The genotype 06V was shown to be responsive to shading, with a statistically equal production independently of shade level. The low, moderate, and high shade levels affected negatively the yield of genotypes 03V and 12V. The shading can improve the yield of Robusta coffee, in this case, of the genotype 06V of the cultivar Conilon Vitória - Incaper 8142. The success of shaded plantations depends the choice of the shade responsive genotypes and appropriate shading level.

O cultivo em sistemas sombreados é uma das estratégias adotadas para mitigar os impactos da mudança climática sobre os cafeeiros. No entanto, existem poucos estudos sobre o efeito do sombreamento no crescimento e produção de clones de café robusta (Coffea canephora). Além disso, existe um paradigma no Brasil de que o sombreamento nesta espécies é sinônimo de menor rendimento. Assim, o objetivo do presente trabalho foi avaliar se os genótipos de C. canephora submetidos ao sombreamento aumentam sua produtividade e crescimento vegetativo em condições de campo. O estudo foi realizado entre setembro de 2013 e julho de 2017 (quatro safras) em Alegre, Espírito Santo, Brasil. Três genótipos da cultivar Conilon Vitória - Incaper 8142 foram avaliados (03V, 06V e 12V) e quatro níveis de sombreamento: pleno sol (testemunha) e baixo,moderado e alto nível de sombreamento, com 34, 48 e 68% de capacidade real de sombreamento, respectivamente, obtidos com o uso de telas de poliolefinas de cor preta; plantas cultivadas em pleno sol foram apenas cercadas. Os níveis de sombreamento testados afetaram a taxa de crescimento e produtividade dasplantas de café Robusta O sombreamento provocou maior expansão foliar em relação ao pleno sol, bem como maior estiolação dos ramos plagiotrópicos e ortotrópicos. Os baixos, moderados e altos níveis de sombreamento afetaram negativamente a produção dos genótipos 03V e 12V. O sombreamento pode melhorar o rendimento do café Robusta, neste caso, do genótipo 06V da cultivar Conilon Vitória - Incaper 8142. O sucesso dasplantações cafeeiras sombreadas depende da escolha dos genótipos responsivos à sombra e do nível apropriado de sombreamento.

Diversity and abundance of green lacewings (Neuroptera: Chrysopidae) in a Conilon coffee plantation in Acre, Brazil

Coffea canephora (Conilon coffee) has great economic importance for the state of Acre, in northern Brazil. The use of insecticides in this crop has increased considerably in recent years. In order to find species of green lacewing (Chrysopidae, Neuroptera) with potential for use in biological pest control in coffee plantations, we sampled green lacewings during one year in an experimental plantation of Conilon coffee in Acre, in order to assess the diversity, abundance and seasonal occurrence of Chrysopidae species. Samplings were carried out weekly using an entomological net. Overall, 1079 specimens of seven green lacewing species were collected: Ceraeochrysa cincta, Ceraeochrysa claveri, Ceraeochrysa cubana, Ceraeochrysa everes, Chrysoperla externa, Leucochrysa (Nodita) cruentata and Leucochrysa (Nodita) marquezi. Ceraeochrysa cubana was the dominant and constant species, comprising 97% of the sampled specimens and occurring in 82% of the weekly samples. The abundance and constant presence of C. cubana, which preys upon several agricultural pests and has shown resistance to various insecticides, make this species a potential candidate for future programs of biological control in Conilon coffee plantations in the southwestern Brazilian Amazon region. (AU)

Aplicação da espectroscopia de infravermelho médio associada à quimiometria para classificação de café verde / Application of medium infrared spectroscopy associated with chemometry for green coffee classification

O agronegócio do café é uma das atividades que mais contribuem para economia brasileira. Objetivou-se avaliar a utilização da espectroscopia no infravermelho médio aliado a análise estatística multivariada para classificar amostras de café. As amostras foram adquiridas em Vitória da Conquista-Bahia e foram utilizadas para obtenção dos espectros no FTIR-ATR – Espectroscopia de Infravermelho com Transformada de Fourier com Reflexão Total Atenuada, sob a faixa espectral de 4000 a 600 cm(-1). Os resultados foram avaliados por meio da estatística multivariada Análise de Componentes Principais - ACP. Na ACP dois componentes explicaram 94,49% da variância total dos dados, onde o primeiro componente principal (CP1) explicou 82,1% e o segundo componente principal (CP2) 12,39%. É possível classificar amostras de café por meio destas ferramentas.

Avaliação da composição de diferentes classes de café verde produzidos no sudoeste da Bahia / Evaluation of the composition of different green coffee classes produced in southwest Bahia

O grão de café é uma das matérias-primas de maior importância comercial. Objetivou-se avaliar a atividade antioxidante, os teores de umidade e cinzas e as propriedades ópticas do grão de café verde. Os grãos de café foram moídos e utilizados para as análises, e os resultados obtidos foram submetidos à Análise de Variância (ANOVA). Através das análises foi possível caracterizar o café como sendo muito seco, com umidade abaixo de 12%. Em relação ao teor de cinzas, as amostras analisadas estavam dentro do limite permitido pela legislação. A classe Mole (M) apresentou maior luminosidade em relação às demais podendo ser classificada como de melhor qualidade. Todas as classes apresentaram capacidade antioxidante. Quanto ao teor de compostos fenólicos totais (CFT) não houve diferença significativa entre as classes. Portanto, foi possível avaliar a composição do café verde.

Avaliação das características físico-químicas de óleo de café torrado (Coffea arabica) durante armazenamento em diferentes temperaturas / Evaluation of physico-chemical characteristics of roasted coffee oil (Coffea arabica) stored at different temperatures

O óleo extraído de café torrado é um produto de altíssimo valor agregado, uma vez que seu valor de mercado é muito superior que a matéria-prima principal, o café. Desse modo, o objetivo deste trabalho foi avaliar as características físico-químicas do óleo de café torrado (Coffea arabica) armazenados sob refrigeração e temperatura ambiente por um período de 12 meses. A acidez não apresentou grande variação devido à degradação da lipase durante o processo de torra dos grãos de café, o índice de peróxidos apresentou um resultado crescente durante o armazenamento, porém, mantendo-se dentro do limite estabelecido pela legislação atual (máximo 15 mEq kg-1). Durante o período de um ano, o óleo de café apresentou qualidade de utilização de acordo com a legislação vigente, independente das condições de armazenamento (6 °C ± 2 °C e 25 °C ± 2 °C).

Sinergias mitigación - adaptación al cambio climático en sistemas de producción de café (Coffea arabica), de Tolima, Colombia / Mitigation - adaptation synergies of climate change of coffee (Coffea arabica) production systems in Tolima, Colombia

Resumen El cambio climático del planeta es generado principalmente por el incremento de las emisiones de gases de efecto invernadero. El Panel Intergubernamental de Cambio Climático (IPCC) ha establecido la mitigación y adaptación como estrategias para enfrentar el cambio climático; sin embargo, ambas estrategias se han evaluado poco en conjunto. Con el objetivo de estudiar las posibles sinergias entre mitigación y adaptación, se evaluaron tres sistemas de producción de café en el municipio de Líbano: sistemas agroforestales (SAF) con Cordia allidora, SAF con plátano y monocultivos con cuatro repeticiones por tratamiento. Como indicador de mitigación se estimó la huella de carbono en la producción y procesamiento del grano; mientras que la adaptación se midió como la diversidad de hormigas presentes. Los SAF con C. allidora favorecen tanto la mitigación como la adaptación al cambio climático, tienen una huella de carbono positiva además, tienen una mayor riqueza de géneros de hormigas (12.81 vs -3.0 vs -6.4 Mg CO2e/ha/año en huella de carbono y 1.3 vs 0.6 vs 0.6 de Índice de Margalef de hormigas en SAF con C. alliodora, SAF con plátano y monocultivo, respectivamente). Los sistemas de producción agrícola, manejados de forma amigable con el medio ambiente y en asocio con árboles nativos, son una buena estrategia para implementar programas de mitigación y adaptación al cambio climático. Incluir árboles nativos favorece económicamente a los productores, e incrementa los lugares de refugio y las condiciones mínimas de sobrevivencia, semejantes a los ecosistemas naturales para que los diferentes grupos de animales logren adaptarse a los cambios.(AU)

Abstract Climate change is mainly generated by an increase of greehouse gas emissions. The Intergovernmental Panel on Climate Change (IPCC) has established mitigation and adaptation as strategies to deal with climate change. However, few studies have been developed jointly. With the purpose of studying the possible synergies between mitigation and adaptation, three coffee production systems in the municipality of Líbano: agroforestry systems (AFS) with Cordia allidora, AFS with plantain and monocultures, with four replications by treatment, were evaluated. As mitigation indicator, the carbon footprint in the coffee production and processing was estimated, whereas the adaptation was measured as the ant diversity. The AFS with C. alliodora favor both mitigation and adaptation to climate change since they have a positive carbon footprint and the greatest richness of ant genus (12.8 vs -3.0 vs -6.4 Mg CO2e/ha/year in carbon footprint and 1.3 vs 0.6 vs 0.6 of Margalef Index of ants in AFS with C. alliodora, AFS with plantain and monoculture, respectively). The agricultural production systems, environmental-friendly managed and associated with native trees, are a good strategy to implement in programs of mitigation-adaptation of climate change. The inclusion of native tree favors the producer's economy and increases the shelter places and the minimal conditions for surviving, similar to natural ecosystems so the different groups of animals for climate change adaptation.(AU)

Multiple resistance to bacterial halo blight and bacterial leaf spot in Coffea spp / Resistência múltipla à mancha aureolada e à mancha foliar bacteriana em Coffea spp

Breeding for genetic resistance is an important method of crop disease management, due to the numerous benefits and low cost of establishment. In this study, progenies of 11 Coffea species and 16 wild C. arabica accessions were tested for their response to Pseudomonas syringae pv. garcae, the causal agent of bacterial halo blight, a widespread disease in the main coffee-producing regions of Brazil and considered a limiting factor for cultivation in pathogen-favorable areas; and also to P. syringae pv. tabaci, causal agent of bacterial leaf spot, a highly aggressive disease recently detected in Brazil. Separate experiments for each disease were carried out in a greenhouse, with artificial pathogen inoculations and ideal moisture conditions for disease development. The results showed that C. canephora, C. congensis, C. eugenioides, C. stenophylla, and C. salvatrix progenies, the wild C. arabica accessions Dilla & Alghe and Palido Viridis, and cultivar IPR 102 contain satisfactory levels of simultaneous resistance against bacterial halo blight and bacterial leaf spot. These results are useful in breeding programs for durable resistance to multiple biotic agents, providing new combinations of resistance alleles by hybridization, as well as for phytopathological studies, to identify infraspecific variability of the pathogens.(AU)

O melhoramento de plantas para resistência genética é um método importante para o manejo de doenças, pelos inúmeros benefícios e baixo custo de implementação. No presente estudo, progênies de 11 espécies de Coffea e 16 acessos selvagens de C. arabica foram testados quanto à resposta a Pseudomonas syringae pv. garcae, agente causal da mancha aureolada, doença disseminada nas principais regiões produtoras de café do Brasil e considerada fator limitante para o cultivo em áreas favoráveis a patógenos; e também para P. syringae pv. tabaci, agente causal da mancha foliar bacteriana, doença altamente agressiva detectada recentemente no Brasil. Experimentos separados para cada doença foram realizados em estufa, por meio da inoculação artificial dos patógenos em condições ideais de umidade para o desenvolvimento das doenças. Os resultados mostraram que as progênies Coffea canephora, C. congensis, C. eugenioides, C. stenophylla e C. salvatrix, além dos acessos selvagens de C. arabica Dilla & Alghe e Palido Viridis e da cultivar IPR 102, possuem níveis satisfatórios de resistência simultânea contra mancha aureolada e mancha foliar bacteriana. Os resultados descritos são úteis em programas de melhoramento para resistência duradoura a múltiplos agentes bióticos, fornecendo novas combinações de alelos de resistência por hibridização, bem como para estudos fitopatológicos, para identificar a variabilidade infraespecífica dos patógenos.(AU)

Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L)

Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars within 8 weeks. The somatic embryos developed into rooted plants with a 100% survival rate upon transfer to the greenhouse.

Ant diversity under three coverages in a Neotropical coffee landscape / Diversidad de hormigas en tres coberturas de un paisaje cafetalero neotropical

Abstract The coffee cultivation has displaced natural vegetation and its associated biological diversity. The present study describes the diversity of ground-dwelling ants on eight plantations where coffee is grown in the shade (SHD), eight plantations where coffee is grown under unshaded coffee (SUN) and four patches of forest (FOS) in a coffee-growing region of Colombia. The research was conducted in transects composed of 12 sampling stations, each of which employed active collection, pitfall traps and litter sifting. Nine habitat variables were analyzed to characterize each site, and these values were related to the diversity of ants and functional groups. A total of 92 morphospecies were collected. Myrmicinae was the subfamily with the highest representation. The sample coverage presented a deficit of less than 5 %. The rank abundance curves exhibited differences in the ant assemblages. The ant communities found in the forest exhibited greater diversity and a larger number of exclusive species than the communities found in locations of other land uses; the SHD and SUN communities were characterized by 80.3 % and 62.3 %, respectively, of the diversity found in the forest communities. The functional similarity index as adapted for guilds by Sørensen reveals a high degree of similarity in the structure of their ant communities, but less so in their composition. The shaded coffee plantations have a richer and more equitable ant fauna than the exposed coffee plantations, reinforcing the idea that shade cultivation favors the establishment of ant fauna and, consequently, ecological functionality.(AU)

Resumen La investigación sobre los ecosistemas de café ha recibido considerable atención en las últimas décadas. El cultivo de café ha desplazado la vegetación natural y su diversidad biológica asociada. Con el objeto de caracterizar la diversidad específica de hormigas del suelo, identificar los grupos funcionales en dos sistemas cafeteros contrastantes y relacionar esta diversidad con algunas características del hábitat, se muestrearon ocho cafetales con sombra (CCS), ocho a libre exposición (CLE) y cuatro parches de bosque en la zona cafetalera del sur de Colombia. Se trabajó en dos transectos con 12 estaciones de muestreo, en cada una se empleó búsqueda activa, trampa de caída y cernido de horajasca. Se evaluaron nueve variables del hábitat para caracterizar cada sitio y relacionar estos valores con la diversidad de hormigas. Se recolectaron 15 666 individuos en 92 morfoespecies, Myrmicinae fue la subfamilia con mayor representación. La cobertura del muestreo presentó un déficit inferior al 5 %. Las curvas de abundancia relativas, mostraron diferencias en el ensamblaje de hormigas. Los bosques presentaron comunidades de hormigas más diversas que las de los otros usos del suelo y mayor número de especies exclusivas; el CCS mantiene el 80.3 % de la diversidad de los bosques frente al 62.3 % de ésta en CLE. En síntesis, la fauna de hormigas en los cafetales con sombra es más rica y equitativa que en los cultivos a libre exposición, reforzando la idea de que la implementación de la sombra favorece el establecimiento de la fauna de hormigas y, por ende, la funcionalidad ecológica.(AU)